Paired-end 150-bp reads sequencing of the RNA library was performed on the MiniSeq platform Illumina. The quantity and quality of the RNA solution was assessed using a Qbit machine and an Agilent 2100 Bioanalyzer Agilent Technologies before library construction and sequencing. An RNA library was then constructed using the SMARTer Stranded Total RNA-Seq kit v. Library preparation and sequencing were carried out at the Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.。
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