近藤昭彦サッカー - サッカー観戦日記

■香川の創価学会信者疑惑

Evaluation of the biodegradability of polyurethane and its derivatives by using lipase-displaying arming yeast. Process engineering and optimization of glycerol separation in a packed-bed reactor for enzymatic biodiesel production. Transesterification of phosphatidylcholine in sn-1 position through direct use of lipase-producing Rhizopus oryzae cells as whole-cell biocatalyst. Comparative profiling analysis of central metabolites in Euglena gracilis under various cultivation conditions. Importance of asparagine residues at positions 13 and 26 on the amino-terminal domain of human somatostatin receptor subtype-5 in signalling. In previous experiments focusing on a single target gene, this system has been reported to work in several plant species, including tomato Solanum lycopersicum L. Engineered bio-nanocapsules, the selective vector for drug delivery system. These results demonstrate the potential of YM as a novel prebiotic that selectively increases B. Spectral changes of lysozyme adsorbed on ultrafine silica particles. Phospholipase D production using immobilized cells of streptoverticillium cinnamoneum. Physicochemical and immunological characterization of hepatitis B virus envelope particles exclusively consisting of the entire L Pre-S1+Pre-S2+S protein. Pretreatment of immobilized Candida antarctica lipase for biodesel fuel production from plant oil. In combination with promoter selection, the novel terminator catalog constitutes a basic toolbox for tuning protein expression levels in metabolic engineering and synthetic biology in P. Using Promoter replacement and selection for loss of heterozygosity to generate an industrially applicable sake yeast strain that homozygously overproduces isoamyl acetate. Concentrations of threonine and lysine-derivative 1,5-diaminopentane increased with an increase of the split ratio into the PPP. Improvement of productivity of active horseradish peroxidase in esherichia coli by coexpression of Dsb proteins. Hydrological Processes, 18, 3133-3146. Enzyme-mediated site-specific antibody-protein modification using a ZZ domain as a linker. Direct production of L-lysine from raw corn starch by Corynebacterium glutamicum secreting Streptococcus bovis α-amylase using cspB promoter and signal sequence. In pure cultures, DKT significantly promoted the growth of Bifidobacterium adolescentis, but not that of Fusobacterium nucleatum or Escherichia coli. Long anchor using Flo1 protein enhances reactivity of cell surface-displayed glucoamylase to polymer substrates. Direct ethanol production from cellulosic materials at high temperature using the thermotolerant yeast Kluyveromyces marxianus displaying cellulolytic enzymes. The product portfolio of the microbe is continuously expanding. Herein, we highlight recent advances in engineering of the microbe into novel cell factories that overproduce these precious molecules from pioneering proofs-of-concept up to industrial productivity. Using dissolved oxygen—stat fed-batch fermentation in a 1-L jar fermenter, we could produce 2. Enzymatic packed-bed reactor integrated with glycerol-separating system for solvent-free production of biodiesel fuel. Homo-D-lactic acid fermentation from arabinose by redirection of phosphoketolase pathway to pentose phosphate pathway in L-lactate dehydrogenase gene-deficient lactobacillus plantarum. Development of an Aspergillus oryzae whole-cell biocatalyst coexpressing triglyceride and partial glyceride lipases for biodiesel production. In eukaryotes, pyruvate produced by glycolysis is used in conversion to ethanol and lactate and in anabolic metabolism in the cytosol, or is transported into the mitochondria for use as a substrate in the tricarboxylic acid TCA cycle. Fadong Li, Xinfabg Song, Changyuan Tang, Akihiko Kondoh, Wanjun Zhang 2008 : Stable isotopic charavterisation of precipitation, soil water and groundwater in Tanhang Mountain, north China. Effect of methanol and water contents on production of biodiesel fuel from plant oil catalyzed by various lipases in a solvent-free system. Direct production of ethanol from raw corn starch via fermentation by use of a novel surface-engineered yeast strain codisplaying glucoamylase and α-Amylase. Kinetic and circular dichroism studies of enzymes adsorbed on ultrafine silica particles. Next-generation sequencing of bacterial 16S rRNA gene revealed a significant increase in the relative abundance of bacteria related to the Bifidobacterium genus in the model after incubation with DKT. Collectively, these findings indicate that E. Over-production of various secretory-form proteins in Streptomyces lividans. Efficient ethanol production from starch through development of novel flocculent yeast strains displaying glucoamylase and co-displaying or secreting α-amylase. Development of efficient protein refolding systems using chaperonins. Moreover, cellobiose transporter genes and the other sugar transporter genes were highly upregulated, and simultaneous uptake of glucose and cellobiose was also observed in the Δ tubB strain. Engineering strategy of yeast metabolism for higher alcohol production. Bioprocessing of Renewable Resources to Commodity Bioproducts, Wiley 2014 Chapter 8, Ethanol production from yeasts, Hasunuma, T. Efficient production of ethanol from raw starch by a mated diploid Saccharomyces cerevisiae with integrated α-amylase and glucoamylase genes. Nguen Viet Luong, Tateishi, R. Displaying non-natural, functional molecules on yeast surfaces via biotin-streptavidin interaction. Evaluation of performance of different surface-engineered yeast strains for direct ethanol production from raw starch. Successful tuning of protein expression levels was shown not only for the reporter gene used to define the catalog but also using betaxanthin production as an example application in pathway flux regulation. Wendisch, Christoph Wittmann Abstract The soil microbe Corynebacterium glutamicum is a leading workhorse in industrial biotechnology and has become famous for its power to synthetise amino acids and a range of bulk chemicals at high titre and yield. Overproduction and purification of lon protease from esherichia coli using maltose binding protein MBP fusion system. Characterization of yeast cell surface displayed Aspergillus oryzae β-glucosidase I with high hydrolytic activity on soy bean isoflavone. Hiroaki Abe, Changyuan Tang, Nozomu Takeuchi, Akihiko Kondoh 2018 : Influence of Seasonal Pumping on Groundwater Sources and Flow System, Nagaoka Plain, Japan. The two edited lines showed significant differences in carotenoid accumulation. Bioconversion using immobilized recombinant flocculent yeast cells carrying a fused enzyme gene in an "intelligent" bioreactor. KRS101 on cellulosic biomass and the production of lipids containing high levels of docosahexaenoic acid. Effect of cereal extracts and cereal fiber on viability of Lactobacillus plantarum under gastrointestinal tract conditions. Development of novel yeast cell surface display system for homo-oligomeric protein by coexpression of native and anchored subunits. Dispersion of coagulated particles by contractile flow to orifice. Mathematical Problems in Engineering, Vol. A simple and immediate method for simultaneously evaluating expression level and plasmid maintenance in yeast. Novel strategy for yeast construction using δ-integration and cell fusion to efficiently produce ethanol from raw starch. Altogether, we found that the terminators have a tunable range of 17-fold. Relationships between molecular states conformation and orientation and activities of a-amylase adsorbed on ultrafine silica particles. Dilinur Aji, Akihiko Kondoh, Changyuan Tang 2008 :Analysis of hydrological changes of lakes and rivers in XinJiang using GIS techniques and remote sensing data. Preparation of Thermus thermophilus holo-chaperonin-immobilized microspheres with high ability to facilitate protein refolding. From transcriptome analysis, the Δ tubB strain exhibited upregulation of both cellulase and hemicellulase genes including some that were not originally induced by cellulose. JSC4 through metabolic profiling and an innovative salinity-gradient strategy. Ahmad Al-Hanbali and Akihiko Kondoh 2008 :Groundwater vulnerability assessment and evaluation of human activity impact HAI within the Dead Sea groundwater basin, Jordan. Whole cell biocatalyst for biodiesel fuel production utilizing rhizopusoryzae cells immobilized within biomass support particles. Moreover, a positive correlation was observed between the relative ratio with or without YM administration of B. Production of bionanocapsules in immobilized insect cell culture using porous biomass support particles. Construction of system for localization of target protein in yeast periplasm using invertase. Youngjoo Kwak and A Kondoh 2010 A study on the assessment of multi-factors affecting urban floods using satellite image; a case study in Nakdong basin, S. Wei Yang, Hideki Kobayashi, Xuehong Chen, Kenlo Nishida Nasahara, Rikie Suzuki, Akihiko Kondoh 2017 : Modeling three-dimensional forest structures to drive canopy radiative transfer simulations of bidirectional reflectance factor, International Journal of Digital Earth, DOI:10. The findings also provide insights that might be useful for the development of novel functional foods. Improved production of homo-D-lactic acid via xylose fermentation by introduction of xylose assimilation genes and redirection of the phosphoketolase pathway to the pentose phosphate pathway in L-lactate dehydrogenase gene-deficient Lactobacillus plantarum. Biotinylated bionanocapsules for displaying diverse ligands toward cell-specific delivery. However, the reduction of enzyme production caused by carbon catabolite repression CCR has long been a problem. Single-step purification and characterization of MBP maltose binding protein -DnaJ fusion protein and its utilization for structure-function analysis. Review 2012 Bioconversion of lignocellulose-derived sugars to ethanol by engineered Saccharomyces cerevisiae. Co-fermentation of cellobiose and xylose using beta-glucosidase displaying diploid industrial yeast strain OC-2. Adsorption equilibrium in immuno-affinity chromatography with polyclonal and monoclonal antibodies. Engineering proteins to enhance their partition coefficients in aqueous two-phase systems. Continuous production of phospholipase D using immobilized recombinant streptomyces lividans. Display of both N- and C-terminal target fusion proteins on the Aspergillus oryzae cell surface using a chitin-binding module. Biofunctional TiO2 nanoparticle-mediated photokilling of cancer cells using UV irradiation. An in vitro microbiota model was established using fecal inocula collected from nine healthy volunteers, and each model was found to retain operational taxonomic units similar to the ones in the original human fecal samples. Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae. Journal of The Remote Sensing Society of Japan, 20 1 , 9-18. Direct bioethanol production from cellulose by the combination of cellulase-displaying yeast and ionic liquid pretreatment. Atmospheric Environment, 45, 6682-6695. Rot1p of Saccharomyces cerevisiae is a putative membrane protein required for normal levels of the cell wall β-1, 6-glucan. Quantitative and dynamic analyses of G protein-coupled receptor signaling in yeast using Fus1, enhanced green fluorescence protein EGFP , and His3 fusion protein. Efficient fermentation of xylose to ethanol at high formic acid concentrations by metabolically engineered Saccharomyces cerevisiae. Reaction properties of immobilized catalytic antibodies that deprotect acylated Carbohydrates. Construction of a xylose-metabolizing yeast by genome integration of xylose isomerase gene and investigation of the effect of xylitol on fermentation. Akihiko Kondoh and Changyuan Tang 1996 : Link GIS to 3D groundwater model. Development and application of thermo-sensitive immunomicrospheres for antibody purification. Display of active enzymes on the cell surface of escherichia coli using PgsA anchor protein and their application to bioconversion. Development of an enzyme activity screening system for β-glucosidase-displaying yeasts using calcium alginate micro-beads and flow sorting. Nobumitsu Tsunematsu, Hiroaki Kuze, Tomonori Sato, Masamitsu Hayasaki, Feifei Cui, Akihiko Kondoh 2011 : Potential impact of spatial patterns of future atmospheric warming on Asian dust emission. Bacterial 16S rRNA gene sequence analysis showed that YM administration did not change the microbial diversity or composition. Methods in molecular biology, Vol. Site-specific tetrameric streptavidin-protein conjugation using sortase A. Enantioselective transesterification using lipase-displaying yeast whole-cell biocatalyst. Journal of Geographic Information System, 3, 267-278. Affibody-displaying bionanocapsules for specific drug delivery to HER2-expressing cancer cells. In particular, the redirection of pyruvate metabolism shifted from anabolism for biomass production to catabolism for the production of target chemicals. Repeated batch fermentation from raw starch using a maltose transporter and amylase expressing diploid yeast strain. Hydrological Processes, 15, 2195-2202. During dark periods with depressed CO 2 fixation, KOR1 shows rapid carbohydrate degradation together with increased lipid and carotenoid contents. Role of N-terminal 28-amino-acid region of Rhizopus oryzae lipase in directing proteins to secretory pathway of Aspergillus oryzae. Selection of DNA aptamers using atomic force microscopy. PCR-mediated seamless gene deletion and marker recycling in Saccharomyces cerevisiae. Hydrological Processes, 16, 2173-2187. Effect of histidine residues in antigenic sites on pH dependence of immuno-adsorption equilibrium. Improvement of cellulose-degrading ability of a yeast strain displaying trichoderma reesei endoglucanase by recombination of cellulose-binding domains. Construction of a novel detection system for protein-protein interactions using yeast G-protein signaling. Because selection threshold of both ON- and OFF-state selection can be flexibly tuned, the desired selection conditions can be rapidly pinned down for individual directed evolution experiments without a prior knowledge either on the library population. Proceedings of the CEReS International Symposium on remote Sensing of the Atmosphere and validation of Satellite Data, CEReS, Chiba, Japan, pp. Review 2012 Recent developments in yeast cell surface display toward extended applications in biotechnology. Repeated use of whole-cell biocatalysts immobilized within biomass support particles for biodiesel fuel production. Journal of Geographical Science, 12 3 , 295-300. Eukaryotic genetic switches are larger and more complex than prokaryotic ones, complicating the rational design and evolution of them. Here, we present a robust workflow for the creation and evolution of yeast genetic switches. Wei Yang, Hideki Kobayashi, Kenlo Nishida Nasahara, Rikie Suzuki, Akihiko Kondoh 2017 : Quantitative Evaluation of Bitterlich Sampling for Estimating Basal Area in Sparse Boreal Forests and Dense Tropical Forests, , 7, 143-156. Conformational changes in protein molecules upon adsorption on ultrafine particles. Improvement of isoflavone aglycones production using β-glucosidase secretory produced in recombinant Aspergillus oryzae. Affinity selection of target cells from cell surface displayed libraries : a novel procedure using thermo-responsive magnetic nanoparticles. Cocktail δ-integration: a novel method to construct cellulolytic enzyme expression ratio-optimized yeast strains. Journal of the Japan Society of Photogrammetry and Remote Sensing, 34, 2, 6-14. An Improved Method of Lipase Preparation Incorporating Both Solvent Treatment and Immobilization onto Matrix. Preparation of a whole-cell biocatalyst of mutated candida antaretica lipase B mCALB by a yeast molecular display system and its practical properties. Hiroaki Abe, Changyuan Tang, Akihiko Kondoh 2014 : Effect of Urban Aquifer Exploitation on Subsurface Temperature and Water Quality. Surfactant-modified yeast whole-cell biocatalyst displaying lipase on cell surface for enzymatic production of structured lipids in organic media. Deletion of MCD4 involved in glycosylphosphatidylinositol GPI anchor synthesis leads to an increase in β-1,6-glucan level and a decrease in GPI-anchored protein and mannan levels in the cell wall of Saccharomyces cerevisiae. Efficient and direct glutathione production from raw starch using Saccharomyces cervisiae. The disruption of α-tubulin is a promising approach for creating next-generation enzyme-producing strains of T. Advances in Geosciences, 4, 219-227. Also, the tubulin-disrupted Δ tubB strain had high enzyme production and the same enzyme profile even if the strain was cultured in a glucose-containing medium. Glutamate production from β-glucan using endoglucanase-secreting Corynebacterium glutamicum. Enhancement of activity of lipase-displaying yeast cells and their application to optical resolution of RS -1-benzyloxy-3-chloro-2-propyl monosuccinate. Conclusively, our results indicate that the redirection of pyruvate metabolism is a useful strategy in the metabolic engineering of aerobic microorganisms. Preparation and comparative characterization of immobilized Aspergillus oryzae expressing Fusarium heterosporum lipase for enzymatic biodiesel production. Preparation of thermo-sensitive magnetic hydrogel microspheres and application to enzyme immobilization. Development and application of thermo-sensitive magnetic immunomicrospheres for antibody purification. Review 2013 A review of enzymes and microbes for lignocellulosic biorefinery and the possibility of their application to consolidated bioprocessing technology. Improvement of transphosphatidylation reaction model of phospholipase D from Streptoverticillium cinnamoneum. Hydrogeology Journal, 10, 587-599. Biodiesel-fuel production in a packed-bed reactor using lipase-producing rhizopus oryzae cells immobilized within biomass support particles. Immobilization of Protein on monodispersed colloidal silica with poly ethylene glycol spacer and application of the composites to immunological agglutination tests. This study aimed to determine the effect of E. The Future of Cranes and Wetlands, 61-71. Efficient secretory overexpression of Bacillus subtilis pectate lyase in Escherichia coli and single-step purification. Conclusion Trichoderma reesei α-tubulin is involved in the transcription of cellulase and hemicellulase genes, as well as in CCR. Review Gene copy number and polyploidy on products formation in yeast. Production of ethanol as a major byproduct was completely inhibited, and the limited translocation of pyruvate into the mitochondria shifted the metabolism from respiration for energy conversion to the effective production of lactate or 2,3-butandiole, even under aerobic conditions. Yuki Tosaki, Norio Tase, Akihiko Kondoh, Kimikazu Sasa, Tsutomu Takahashi, Yasuo Nagashima 2011 : Distribution of 36Cl in the Yoro River Basin, central Japan and its relation to regional groundwater flow system. Direct and efficient ethanol production from high-yielding rice using a Saccharomyces cerevisiae strain that express amylases. Isolation of novel catalytic antibody clones from combinatorial library displayed on yeast-cell surface. In this research, we used Target-AID technology to target multiple genes related to carotenoid accumulation in tomato. Construction of a novel synergistic system for production and recovery of secreted recombinant proteins by the cell surface engineering. Phospholipase D from Streptoverticillium cinnamoneum: protein engineering and application for phospholipid production. However, how the split ratio of carbon flux between glycolysis and PPP specifically affects the formation of downstream metabolites remains largely unclear. Review Biotechnological production of enantiomeric pure lactic acid from renewable resources: recent achievements, perspectives, and limits. Development of novel cell surface display in Corynebacterium glutamicum using porin. Development of novel whole-cell immunoadsorbents by yeast surface display of IgG-binding domain. Int'l Workshop on Global Change and Terrestrial Environment in Monsoon Asia, 95-98. Structural changes in protein molecules adsorbed on ultrafine silica particles. Sharma, Ryutaro Tateishi, Akihiko Kondoh, Bayaer Wuliangha, and Gegen Tana 2017 : A Detailed and High-Resolution Land Use and Land Cover Change Analysis over the Past 16 Years in the Horqin Sandy Land, Inner Mongolia. Gγ recruitment system incorporating a novel signal amplification circuit to screen transient protein-protein interactions. Disruption of a typical transcriptional regulator, Cre1, does not sufficiently suppress this reduction in the presence of glucose. Immunological agglutination behavior of latex particles with covalently immobilized antibodies. The non-conventional yeast Pichia pastoris syn. Development of a novel aptamer-based sensing system using atomic force microscopy. Nanoparticles for the delivery of genes and drugs to human hepatocytes. Improvement of ethanol productivity during xylose and glucose co-fermentation by xylose-assimilating S. High-efficiency recovery of target cells using improved yeast display system for detection of protein-protein interactions. This is the first report of overcoming CCR by disrupting α-tubulin gene in T. Separation of murine neutrophils and macrophages by thermoresponsive magnetic nanoparticles. Review Protein-protein interactions and selection introduction. Water activity dependence of performance of surface-displayed lipase in yeast cells: A unique water requirement for enzymatic synthetic reaction in organic media. KOR1 was subsequently characterized with extensive mutation of the ISA1 gene encoding a starch debranching enzyme DBE. Review 2012 Enzyme-mediated methodologies for protein modification and bioconjugate synthesis. These results suggested that the Δ tubB strain was released from CCR. PCC 6803, probed by time-resolved fluorescence spectroscopy. Additionally, in pure cultures, B. Review 2013 Endowing non-cellulolytic microorganisms with cellulolytic activity aiming for consolidated bioprocessing. Atsushi Higuchi, Akihiko Kondoh and Shinkichi Kishi 1997 :Ground based observation of the rice pady canopy using the visible-near infrared video camera in BIWAKO Project'96, Japan. The rational enzyme design of the substrate-binding site by computational simulation improved 1,3-butadiene-producing ccMA decarboxylation with a small mutant library. This study constructed a novel artificial metabolic pathway for 1,3-butadiene production from glucose in Escherichia coli by combining the cis,cis-muconic acid ccMA -producing pathway and tailored ferulic acid decarboxylase mutants. Efficient production of optically pure D-lactic acid from raw corn starch using a genetically modified L-lactate dehydrogenase gene-deficient and α-amylase-secreting Lactobacillus plantarum. Review 2013 Development of microbial cell factories for bio-refinery through synthetic bioengineering. Balasubramanian, and Kondoh, A. Over-expression system for secretoryphospholipase D by streptomyceslividans. Application of magnetic nanoparticles in full-automated chemiluminescent enzyme immunoassay. Efficient production of L- + -lactic acid from raw starch by Streptococcus bovis 148. Komagataella phaffii has frequently been used as a platform for metabolic engineering but knowledge regarding P. Hydrological Processes, 16, 2805-2817. KRS101 using a mutant allele of the gene encoding ribosomal protein L44 as a selectable transformation marker for cycloheximide resistance. Review Applications of yeast cell-surface display in bio-refinery. Capabilities of polymer-modified monodisperse colloidal silica particles as biomaterial carrier. Use of mono-and diacylglycerol lipase as immobilized fungal whole cells to convert residual partial glycerides enzymatically into fatty acid methyl esters. Wei Yang, Akihiko Kondoh 2016 : Toward Circumventing Collinearity Effect in Nonlinear Spectral Mixture Analysis by Using a Spectral Shape Measure,, 5, 185-193. Direct ethanol production from cassava pulp using a surface-engineered yeast strain co-displaying two amylases, two cellulases, and β-glucosidase. Proceedings of International workshop on Macro-Scale Hydrological Modeling, Hohai University Press, 124-127. Environmental Research Center Papers, No. Circular dichroism studies on conformational changes in protein molecules upon adsorption on ultrafine polystyrene particles. Metabolomic and transcriptomic analyses showed an emphasis on glycolysis and a repressed TCA cycle. The in vitro model simulating human colonic microbiota revealed that E. Isolation of a novel promoter for efficient protein expression by Aspergillus oryzae in solid-state culture. Review Protein-protein interactions and selection: yeast-based approaches that exploit guanine nucleotide-binding protein signaling. Adsorption of g-globulin, a model protein for antibody, on colloidal particles. Improvement in lactic acid production from starch using α-amylase-secreting lactococcus lactis cells adapted to maltose or starch. Proceedings of International workshop on Macro-Scale Hydrological Modeling, Hohai University Press, 94-97. Variation in biomass properties among rice diverse cultivars.。

近藤 昭彦 サッカー - サッカー観戦日記

■香川の創価学会信者疑惑

近藤昭仁

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近藤 昭彦 サッカー

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近藤 昭彦 サッカー

■香川の創価学会信者疑惑

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近藤昭彦サッカー

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